![55. Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA](https://cdn.prod.website-files.com/6380be6ed1140b78394d35e6/6481dd731d80e4da40346921_WeldonNCB_2016.jpeg)
RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.